3 Tips For Designing More Effective Peptides

Business Blog

If you are in charge of designing peptides for your workplace, here are three tips that will help design more effective peptides.

#1 Minimal Length

To start with, you need to watch the sequence length of your peptides. When your peptides start to get really long, the purity and yield that you get from your peptides start to decrease and they become less cost effective. As the length of your peptide gets longer, the purity that you get from the peptide decreases, so whenever possible, stick to short peptides. If you need to use longer peptides, watch the purity levels carefully.

#2 Limit Oxidation

The next thing you need to consider is the potential for oxidation for the peptides that you create. There are certain residues that are more likely to oxidation within your peptide. When this happens the purity of your peptide will be impacted negatively. The solubility will be negatively impacted as well.

Stick to peptides such as Nerleucine and Ser, and stay away from peptides such as Mets, Trp and Cys, all of which increase the chance that your peptide will experience oxidation and will not be as pure or as soluble as you need it to be. If you need to use Mets, Trp and Cys, try to limit its usage in your peptides to decrease the chance of negative side effects on the purity, oxidation and solubility it will have on your peptides.  

#3 Right Solubility

Finally, you are going to want to make sure that you design peptides that have the solubility that you need. There are a few things that you can do to ensure that your peptide has the right solubility. First, you can make sure that your peptide doesn't include long strings of amino acids. Second, you are going to want to make sure that you have the right ratio of charged to uncharged residues.  A larger ratio is generally preferred. Finally, make sure that you do not use too many hydrophilic connections in your peptide; this can affect the overall purity of your peptide, even if it is short enough.

Creating peptides is a complicated process which can be controlled by examining the peptides that you are creating and thinking about how each design element will affect the overall usability of the peptide. Focus on minimizing the length, limiting oxidation and creating the right solubility when you design your next set of peptides. If you need help, contact a business such as Maxim Peptide. 

22 February 2017